Optimization of Trissolcus japonicus cold storage methods for biological control of Halyomorpha halys

Trissolcus japonicus (Ashmead) (Hymenoptera: Scelionidae) has shown promise as a biological control agent for the invasive agricultural and nuisance pest, Halyomorpha halys (Stål) (Hemiptera: Pentatomidae). In addition to need laboratory research colonies, possibility rearing T. classical or augmentative releases necessitates investigation into efficient protocols with over timing of production. Cold storage is means slow insects’ metabolism cold temperatures can be used manipulate when how many parasitoids are available use. Conditions (i.e., temperature photoperiod), duration exposure cold, nutrition, age, sex all affect success regime. We tested these factors affected mortality, realized fertility females, progeny, development time cold-stored adult immature using previously frozen H. egg masses. Rearing at 8, 13, 18 °C (10:14 h L:D) significantly increased longevity compared 23 (16:8 L:D); however, females reared 8 °C, 10:14 L:D, we found sublethal effects on their progeny. Adults access food survived greater levels than those without food. high mortality immatures in ages conditions tested. Additionally, differences emergence from fresh eggs was assessed. This work compare costs benefits will assist forecasting after stored.